Thermo Scientific™

SuperSignal™ West Dura Extended Duration Substrate

Thermo Scientific SuperSignal West Dura Extended Duration Substrate is a luminol-based enhanced chemiluminescence (ECL) horseradish peroxidase (HRP) substrate ideal for자세히 알아보기

Thermo Scientific SuperSignal West Dura Extended Duration Substrate is a luminol-based enhanced chemiluminescence (ECL) horseradish peroxidase (HRP) substrate ideal for quantitative western blotting with stable light output for mid-femtogram level detection for western blot analysis.

SuperSignal West Dura Extended Duration Substrate characteristics include:
• Sensitivity: mid-femtogram
• Stability: 24-hr working solution stability; 1-year kit stability at room temperature
• Compatibility: nitrocellulose and PVDF membranes
• Signal duration: 24 hours
• Recommended primary antibody concentration: 1:1,000–1:50,000 dilution (0.02–1.0 μg/mL)
• Recommended secondary antibody concentration: 1:50,000–1:250,000 dilution (4–20 ng/mL)

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SuperSignal West Dura Extended Duration Substrate for HRP is optimized for high sensitivity and long signal duration, making it ideal for cooled CCD camera-based detection systems. Unlike substrates with signals that decline to barely detectable levels in 30–60 minutes, the signal produced with SuperSignal West Dura chemiluminescent substrate is stable for 24 hours, allowing multiple film or camera exposures.

사양

제품 유형Substrate

검출 방법Chemiluminescence

형태Liquid

용도(애플리케이션)Western Blot

Membrane CompatibilityNitrocellulose, PVDF

제품라인SuperSignal™

수량100 mL

SubstrateHorseradish Peroxidase

권장 항체 농도2°: 1:50-250K (4-20ng/mL), 1°: 1:5K (0.02- 1μg/mL)

민감도Mid-femtogram

신호 지속 시간24 hr.

충분12 Mini-Gel Blots, 1000 sq. cm of Membrane

키트 구성품Luminol/Enhancer, 50mL, Stable Peroxide Buffer, 50mL

Unit SizeEach