Thermo Fisher Scientific AMPK alpha-2 Polyclonal Antibody
AMPK alpha-2 단백질을 인식하는 Rabbit Polyclonal Antibody로, WB, IHC, IP 등 다양한 응용에 사용 가능. 인간, 생쥐, 랫드에 반응하며 높은 특이성과 민감도를 제공. 에너지 대사 관련 연구에 적합.
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Thermo Fisher Scientific AMPK alpha-2 Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:3,000 | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 | - |
| Immunoprecipitation (IP) | 1:100–1:500 | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fragment corresponding to a region within amino acids 29–510 of AMPK alpha 2 (Uniprot ID: P54646) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.96 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 20% glycerol |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_11153585 |
Product Specific Information
- Recommended positive controls: 293T, A431, HeLa, HepG2, NIH-3T3
- Predicted reactivity: Mouse (98%), Rat (98%), Pig (99%), Chicken (96%), Sheep (98%), Bovine (99%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
The protein encoded by this gene is a catalytic subunit of AMP-activated protein kinase (AMPK).
AMPK is a heterotrimer consisting of an alpha catalytic subunit and non-catalytic beta and gamma subunits.
AMPK functions as an energy-sensing enzyme that monitors cellular energy status.
Upon activation under metabolic stress, AMPK phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and HMG-CoA reductase (HMGCR), key enzymes in fatty acid and cholesterol biosynthesis.
Mouse studies indicate that this subunit may regulate insulin sensitivity and maintain myocardial energy homeostasis during ischemia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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