Thermo Fisher Scientific resDNASEQ dPCR E. coli DNA Kit

Applied Biosystems™ resDNASEQ™ dPCR E. coli DNA Kit

The Applied Biosystems resDNASEQ dPCR E. coli DNA Kit is a digital PCR (dPCR)-based solution designed to detect host-cell DNA from E. coli, an expression system commonly used for recombinant protein and plasmid production. Reliable and rapid, the assay enables sensitive and specific quantification of E. coli DNA, ensuring confidence in quantitation data across a broad range of sample types—from in-process samples with various matrices to final product formulations.

Features

• Accurate, absolute quantitation of residual host-cell E. coli DNA
• Highly sensitive quantitation using proven TaqMan Assays, with results in under three hours
• Includes a highly characterized E. coli DNA control for qualification and verification
• Easy-to-use, integrated sample-to-results kit: Absolute Q DNA Digital PCR Master Mix, E. coli assay mix, DNA positive control, and DNA dilution buffer
• Supports manual or automated sample preparation optimized for quantitative recovery from complex matrices

Integrated Workflow Solution

The resDNASEQ dPCR E. coli DNA Kit is part of an integrated workflow for impurity and contaminant testing during biopharmaceutical manufacturing.
Validated on the QuantStudio Absolute Q Digital PCR System, which tolerates common inhibitors in biopharmaceutical sample matrices—sometimes eliminating the need for nucleic acid extraction.
If extraction is required, the PrepSEQ Residual DNA Sample Preparation Kit is recommended, offering both manual and automated workflows.

Easy to Use

With the resDNASEQ dPCR E. coli DNA Kit, dPCR analysis quantifies copy numbers of residual E. coli DNA in test samples.
The kit provides essential reagents—from master mix and TaqMan primer/probe mix to genomic DNA control—along with step-by-step instructions.
A copy-to-mass conversion tool is also included to assist with regulatory compliance.

제품 사양