Thermo Fisher Scientific Nhe-1 Polyclonal Antibody
인간 Nhe-1 단백질을 인식하는 Rabbit Polyclonal 항체로, IHC(P) 실험에 적합합니다. 항원 친화 크로마토그래피로 정제되었으며, PBS 버퍼와 글리세롤로 안정화되어 있습니다. 세포 내 pH 조절 및 세포골격 연구에 활용 가능합니다.
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Applications
- Immunohistochemistry (Paraffin) (IHC (P)): 1:200–1:500
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human Nhe-1. Recombinant protein control fragment (Product #RP-103029) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2644660 |
Product Specific Information
Immunogen sequence:
KILRNNLQKT RQRLRSYNRH TLVADPYEEA WNQMLLRRQK ARQLEQKINN YLTVPAHKLD SPTMSRARIG SDPLAYEPKE DLPVITIDPA
- Highest antigen sequence identity to orthologs:
- Mouse: 96%
- Rat: 97%
Target Information
The Na⁺/H⁺ antiporter (Nhe-1) is a ubiquitous membrane-bound enzyme involved in pH regulation of vertebrate cells. It is specifically inhibited by the diuretic drug amiloride and activated by various signals including growth factors, mitogens, neurotransmitters, and tumor promoters. Nhe-1 also acts as an anchor for actin filaments, maintaining cortical cytoskeleton integrity through interactions with ERM proteins (ezrin, radixin, moesin). It may play structural roles in cytoskeletal regulation independent of ion exchange and is implicated in hypertension. At least two isoforms of Nhe-1 are known.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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