Thermo Fisher Scientific MCM3 Polyclonal Antibody
Rabbit polyclonal antibody targeting human MCM3 protein. Western blot, ELISA, IP에 적합하며 Human, Mouse, Rat에 반응. 핵 내 단백질 검출용으로 연구 전용. -20°C에서 보관, 동결-해동 반복 피해야 함.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| ELISA | 1 µg/mL |
| Immunoprecipitation (IP) | 0.5–4 µg antibody for 200–400 µg extracts |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fusion protein corresponding to amino acids 1–295 of human MCM3 (NP_002379.2) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.17 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2804589 |
Product Specific Information
Immunogen sequence:
MAGTVVLDDV ELREAQRDYL DFLDDEEDQG IYQSKVRELI SDNQYRLIVN VNDLRRKNEK RANRLLNNAF EELVAFQRAL KDFVASIDAT YAKQYEEFYV GLEGSFGSKH VSPRTLTSCF LSCVVCVEGI VTKCSLVRPK VVRSVHYCPA TKKTIERRYS DLTTLVAFPS SSVYPTKDEE NNPLETEYGL SVYKDHQTIT IQEMPEKAPA GQLPRSVDVI LDDDLVDKAK PGDRVQVVGT YRCLPGKKGG YTSGTFRTVL IACNVKQMSK DAQPSFSAED IAKIKKFSKT RSKDI
Positive Samples: HeLa, 293T, PC-12, NIH/3T3, COS-1, COS-7
Cellular Location: Nucleus
Target Information
The MCM3 protein is one of the highly conserved mini-chromosome maintenance proteins involved in the initiation of eukaryotic genome replication. The hexameric MCM2–7 complex forms a key component of the pre-replication complex (pre_RC) and participates in replication fork formation and recruitment of other DNA replication-related proteins. MCM3 interacts with MCM5/CDC46 and is acetylated by MCM3AP, a chromatin-associated acetyltransferase. Acetylation inhibits DNA replication initiation and cell cycle progression.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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