Thermo Fisher Scientific BrdU Monoclonal Antibody (OTI1C7), TrueMAB
BrdU 검출용 단클론 항체로, 세포 증식 연구에 활용 가능. IHC(P) 및 ICC/IF에서 1:150 희석 권장. 마우스 IgG1 항체이며, 동결건조 형태로 제공. DNA 복제 중 BrdU 삽입 세포를 특이적으로 검출 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
| Immunocytochemistry (ICC/IF) | 1:150 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Chemical |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1C7 |
| Immunogen | Bromodeoxyuridine coupled to keyhole limpet hemocyanin (KLH) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
Bromodeoxyuridine (5-bromo-2-deoxyuridine, BrdU) is a synthetic nucleoside analogue of thymidine.
It is used to detect proliferating cells in living tissues by incorporating into newly synthesized DNA during the S phase of the cell cycle.
Antibodies specific for BrdU enable detection of incorporated BrdU, indicating actively replicating cells.
Binding of the antibody requires DNA denaturation, typically achieved by acid or heat treatment.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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