Thermo Fisher Scientific BrdU Monoclonal Antibody (OTI1C7), TrueMAB

Applications and Tested Dilution

Product Specifications

Product Specific Information

For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).

Target Information

Bromodeoxyuridine (5-bromo-2-deoxyuridine, BrdU) is a synthetic nucleoside analogue of thymidine.
It is used to detect proliferating cells in living tissues by incorporating into newly synthesized DNA during the S phase of the cell cycle.
Antibodies specific for BrdU enable detection of incorporated BrdU, indicating actively replicating cells.
Binding of the antibody requires DNA denaturation, typically achieved by acid or heat treatment.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.