Thermo Fisher Scientific SMAD1 Monoclonal Antibody (7G11G7)
SMAD1 단백질을 인식하는 Mouse monoclonal antibody로, Western blot, IHC, ICC, Flow cytometry, ELISA 등에 사용 가능. Human 및 비인간 영장류 반응성. Protein G 정제, 1 mg/mL 농도, PBS buffer에 0.05% sodium azide 포함. 연구용 전용 제품.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (IHC) | Assay-dependent |
| Immunocytochemistry (ICC/IF) | Assay-dependent |
| Flow Cytometry (Flow) | 1:200–1:400 |
| ELISA | 1:10,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Non-human primate |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 7G11G7 |
| Immunogen | Purified recombinant fragment of human SMAD1 (AA: 1–110) expressed in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_3090525 |
Target Information
The protein encoded by this gene belongs to the SMAD family, which includes proteins similar to the Drosophila gene mothers against decapentaplegic (Mad) and the C. elegans gene Sma. SMAD proteins act as signal transducers and transcriptional modulators mediating multiple signaling pathways.
SMAD1 mediates the signals of bone morphogenetic proteins (BMPs), which regulate cell growth, apoptosis, morphogenesis, development, and immune responses. Upon BMP ligand binding, SMAD1 is phosphorylated and activated by BMP receptor kinases. The phosphorylated SMAD1 forms a complex with SMAD4 to regulate transcription.
SMAD1 is targeted by SMAD-specific E3 ubiquitin ligases such as SMURF1 and SMURF2, leading to ubiquitination and proteasome-mediated degradation. Alternatively spliced transcript variants encoding the same protein have been observed.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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