Thermo Fisher Scientific SILAC Protein Quantitation Kit (LysC), DMEM:F-12

Thermo Fisher Scientific SILAC Protein Quantitation Kit (LysC), DMEM:F-12

The Thermo Scientific™ SILAC Protein Quantitation Kit - DMEM:F12 is designed for specific analysis of protein expression by mass spectrometry using stable isotope labeling with amino acids in cell culture (SILAC).

Features

• Efficient: Up to 100% label incorporation into proteins of living cells
• Reproducible: Minimizes intra-experimental variability caused by differential sample preparation
• Flexible: Media deficient in both L-lysine and L-arginine for more complete proteome coverage through dual amino acid isotope labeling
• Compatible: Suitable for labeling proteins in various mammalian cell lines adapted to DMEM:F12 medium, including HeLa, 293T, COS7, U2OS, A549, A431, HepG2, NIH 3T3, Jurkat, and others
• High-quality supplements: Heavy amino acids with >99% isotope purity; dialyzed FBS tested to ensure sterility, endotoxin-free, and cell culture compatibility

Applications

• Quantitative analysis of relative changes in protein abundance from different cell treatments
• Quantitative analysis of proteins lacking specific antibodies
• Protein expression profiling for studying normal vs. disease cells
• Identification and quantification of hundreds to thousands of proteins in a single experiment

Description

SILAC enables in vivo metabolic incorporation of heavy (^13C or ^15N) labeled amino acids into proteins, followed by mass spectrometry (MS) for comprehensive identification and quantitation.
Because peptides labeled with heavy and light amino acids are chemically identical, they co-elute during reverse-phase chromatography and are detected simultaneously during MS analysis. The relative peak intensities of isotopically distinct peptides are used to determine changes in protein abundance.

Each kit includes all necessary reagents to isotopically label cells with ^13C₆ L-lysine-2HCl, including media, heavy and light amino acids, and dialyzed serum.
Heavy L-arginine-HCl derivatives (Cat. Nos. 88210, 89990) are available separately and can be combined with Pierce SILAC Protein Quantitation Kits for enhanced peptide isotope label coverage.

When used with Thermo Scientific Protein/Peptide Sample Enrichment Products, this kit also enables MS analysis of low-abundance proteins, such as cell surface or organelle-specific proteins, and post-translational modifications (e.g., phosphorylation, glycosylation).

Related Products

• Dialyzed Fetal Bovine Sera, US Origin
• Pierce Trypsin Protease, MS Grade
• DMEM:F-12 for SILAC

Specifications