Thermo Fisher Scientific VCP Monoclonal Antibody (Hs-14)
VCP 단백질을 인식하는 Hs-14 클론의 마우스 단클론 항체로, WB, ICC/IF, Flow Cytometry에 사용 가능. 인간 및 생쥐 반응성. 비결합형 액상 형태로 4°C 보관. 세포 내막 투과 염색에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | Assay-dependent |
| Immunocytochemistry (ICC/IF) | 10 µg/mL |
| Flow Cytometry (Flow) | 3–12 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Mouse / IgM |
| Class | Monoclonal |
| Type | Antibody |
| Clone | Hs-14 |
| Immunogen | Freshly ejaculated human sperms were washed in PBS and extracted in 3% acetic acid, 10% glycerol, 30 mM benzamidine. The acid extract was dialyzed against 0.2% acetic acid and subsequently used for immunization. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage Buffer | TBS, pH 8.0 |
| Contains | 15 mM sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Wet ice |
| RRID | AB_11156636 |
Additional Formats
Product Specific Information
This antibody reacts with VCP (valosin-containing protein), a 220 kDa intra-acrosomal protein.
For ICC staining, membrane permeabilization with acetone is essential.
Target Information
The protein encoded by this gene is a member of a family that includes putative ATP-binding proteins involved in vesicle transport and fusion, 26S proteasome function, and assembly of peroxisomes.
This protein, as a structural protein, is associated with clathrin and heat-shock protein Hsc70 to form a complex.
It has been implicated in several cellular events regulated during mitosis, including homotypic membrane fusion, spindle pole body function, and ubiquitin-dependent protein degradation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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