Thermo Fisher Scientific VCP Monoclonal Antibody (Hs-14)

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-Dependent

-

Immunocytochemistry (ICC/IF)

10 µg/mL

-

Flow Cytometry (Flow)

3-12 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Mouse / IgM

Class

Monoclonal

Type

Antibody

Clone

Hs-14

Immunogen

Freshly ejaculated human sperms were washed in PBS and extracted in 3% acetic acid, 10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0.2% acetic acid and subsequently used for immunization. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:VCP,uniProtId:P55072-1,ncbiNodeId:9606,antigenRange:1-806,antigenLength:806,antigenImageFileName:MA1-10383_VCP_P55072-1_House_mouse.svg,antigenImageFileNamePDP:MA1-10383_VCP_P55072-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

View additional formats

• FITC
• Request custom conjugation

Form

Liquid

Concentration

1 mg/mL

Purification

sequential chromatography

Storage buffer

TBS, pH 8.0

Contains

15mM sodium azide

Storage conditions

4° C, do not freeze

Shipping conditions

Wet ice

RRID

AB_11156636

Product Specific Information

This antibody reacts with VCP (valosin-containing protein) a 220 kDa intra-acrosomal protein.

For ICC staining, membrane permeabilization with acetone is essential.

Target Information

The protein encoded by this gene is a member of a family that includes putative ATP-binding proteins involved in vesicle transport and fusion, 26S proteasome function, and assembly of peroxisomes. This protein, as a structural protein, is associated with clathrin, and heat-shock protein Hsc70, to form a complex. It has been implicated in a number of cellular events that are regulated during mitosis, including homotypic membrane fusion, spindle pole body function, and ubiquitin-dependent protein degradation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.