Thermo Fisher Scientific H3K9acK14ac Polyclonal Antibody
H3K9acK14ac 폴리클로날 항체로 인간 및 생쥐 시료에 반응. Western blot, ICC/IF, Dot blot 등 다양한 응용 가능. 항원 친화 크로마토그래피로 정제된 고순도 제품. 액상 형태로 제공되며 장기 보관 시 -20°C 권장. 연구용 전용 시약.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:5,000–1:20,000 |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 |
| Dot blot (DB) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic acetyl-peptide corresponding to amino acids surrounding Acetyl Lys9 and Lys14 of Histone H3 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 1% BSA, 20% glycerol |
| Contains | 0.01% thimerosal |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2549501 |
Product Specific Information
- Recommended positive controls: 293T, A431, HeLa, HepG2, NIH-3T3, JC, BCL-1, HeLa (0.4 µM Trichostatin treatment for 18 hr)
- Store product as a concentrated solution.
- Centrifuge briefly prior to opening the vial.
Target Information
Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3, along with four core histone proteins, binds to DNA forming the nucleosome structure. Histones play a central role in transcription regulation, DNA repair, DNA replication, and chromosomal stability. Post-translational modifications of histones can alter chromatin structure or facilitate transcription factor binding. The N-terminal tail of histone H3 can undergo methylation, acetylation, and phosphorylation, which influence various cellular processes.
⚠ WARNING: This product can expose you to chemicals including mercury, known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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