Thermo Fisher Scientific PCMT1 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

0.04-0.4 µg/mL

-

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:500

-

Immunocytochemistry (ICC/IF)

0.25-2 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein corresponding to Human PCMT1. Recombinant protein control fragment (Product #RP-96782). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:PCMT1,uniProtId:P22061-1,ncbiNodeId:9606,antigenRange:80-224,antigenLength:227,antigenImageFileName:PA5-51872_PCMT1_P22061-1_Rabbit.svg,antigenImageFileNamePDP:PA5-51872_PCMT1_P22061-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2645288

Product Specific Information

Immunogen sequence: AKALDVGSGS GILTACFARM VGCTGKVIGI DHIKELVDDS VNNVRKDDPT LLSSGRVQLV VGDGRMGYAE EAPYDAIHVG AAAPVVPQAL IDQLKPGGRL ILPVGPAGGN QMLEQYDKLQ DGSIKMKPLM GVIYVPLTDK EKQWS

Highest antigen sequence identity to the following orthologs: Mouse - 94%, Rat - 94%.

Target Information

Three classes of protein carboxyl methyltransferases, distinguished by their methyl-acceptor substrate specificity, have been found in prokaryotic and eukaryotic cells. The type II enzyme catalyzes the transfer of a methyl group from S-adenosyl-L-methionine to the free carboxyl groups of D-aspartyl and L-isoaspartyl residues. These methyl-accepting residues result from the spontaneous deamidation, isomerization, and racemization of normal L-aspartyl and L-asparaginyl residues and represent sites of covalent damage to aging proteins PCMT1 (EC 2.1.1.77) is a protein repair enzyme that initiates the conversion of abnormal D-aspartyl and L-isoaspartyl residues to the normal L-aspartyl form.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.