Thermo Fisher Scientific MYPT1 Polyclonal Antibody
Rabbit polyclonal antibody targeting human MYPT1 (PPP1R12A). Validated for WB and IHC-P. Supplied as liquid, antigen affinity purified, unconjugated. Recommended for research use in studying myosin phosphatase signaling pathways.
- 판매단위
- pk
카탈로그
1개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific MYPT1 Polyclonal Antibody
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.04–0.4 µg/mL |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:20–1:50 |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human PPP1R12A. Recombinant protein control fragment (Product # RP-105553) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.05 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2792033 |
Product Specific Information
Immunogen sequence:
DILGYLEELQ KKQNLLHSEK RDKKSPLIES TANMDNNQSQ KTFKNKETLI IEPEKNASRI ESLEQEKVDE EEEGKKDESS CS
Target Information
Myosin phosphatase target subunit 1 (MYPT1), also known as the myosin-binding subunit of myosin phosphatase, is a regulatory component of myosin phosphatase that modulates actin–myosin interactions downstream of the small GTPase Rho.
Activated RhoA (GTP·RhoA) interacts with the myosin-binding subunit (MBS) of myosin phosphatase, influencing phosphorylation of the myosin light chain (MLC). Rho-associated kinase (Rho-kinase), activated by GTP·RhoA, phosphorylates and inactivates myosin phosphatase. Overexpression or activation of RhoA in NIH 3T3 cells increases MBS and MLC phosphorylation, suggesting Rho inhibits myosin phosphatase through Rho-kinase activity. Multiple transcript variants encoding different isoforms have been identified for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.