Thermo Fisher Scientific RapidFinder Quant Multi-Animal Set

Thermo Scientific™ RapidFinder™ Quant Multi-Animal Set

The RapidFinder™ Quant Multi-Animal Set, when used with one or more of the RapidFinder™ Animal Species ID kits listed below, enables the determination of the percentage of a target animal species with respect to total animal DNA in food and feed samples. Using the included standards, the kit allows quantification of as little as 0.05% target species DNA compared to other animal DNA within a few hours.

Compatible ID Kits (Sold Separately)

주요 특징

• Simple real-time PCR based assay, no electrophoresis needed
• Flexible: compatible with any food or feed sample, both raw and processed
• Reliable: includes multi-animal DNA quantification standard
• Fast: from sample to result within the same day

How It Works

The method relies on quantitative real-time PCR. A standard curve is created by serial dilution of the included multi-animal DNA standard. Assays for the target species DNA (from one of the listed kits) and total animal DNA (from this set) are run side-by-side on both food sample DNA and DNA standards. This allows rapid and accurate quantification of the target animal species percentage relative to total animal DNA.
Each reaction mix includes an internal positive control detected in the VIC™ dye channel to verify the absence of PCR inhibitors.

The RapidFinder™ Quant Multi-Animal Set is optimized and validated for use with the Applied Biosystems™ QuantStudio™ 5 Real‑Time PCR Instrument and the Applied Biosystems™ 7500 Fast Real‑Time PCR Instrument.

Sample Preparation

DNA sample preparation for the RapidFinder™ animal species identification and quantification workflow is achieved using the GMO Extraction Kit (Cat. No. 4466336). Using 10 g of sample helps minimize sampling errors. The kit is compatible with raw and processed meat, as well as composite food samples.

사양 (Specifications)