Thermo Scientific™

RapidFinder™ Quant Multi-Animal Set

The RapidFinder™ Quant Multi-Animal Set, when used with one or more of the RapidFinder™ Animal Species ID kits listed below,자세히 알아보기

The RapidFinder™ Quant Multi-Animal Set, when used with one or more of the RapidFinder™ Animal Species ID kits listed below, enables the determination of the percentage of a target animal species with respect to total animal in food and feed samples. Using the included standards, the kit enables quantification of as little as 0.05% target species DNA as compared to other animal DNA in only a few hours.

The RapidFinder Quant Multi-Animal Set is designed to be used in combination with one or more of the following animal species-specific ID kits (available separately):

Name	Catalog Number
RapidFinder™ Beef ID Kit	A24391
RapidFinder™ Pork ID Kit	A24392
RapidFinder™ Equine ID Kit	A15570
RapidFinder™ Poultry ID Kit	A24397
RapidFinder™ Chicken ID Kit	A24393
RapidFinder™ Turkey ID Kit	A24394
RapidFinder™ Halal ID Kit	A47086
RapidFinder™ Sheep ID Kit	A24395
RapidFinder™ Goat ID Kit	A24407
RapidFinder™ Fallow Deer ID Kit	A24410

• Simple-real-time PCR based assay, no electrophoresis needed
• Flexible-compatible with any food or feed sample, both raw and processed
• Reliable-Includes multi-animal DNA quantification standard
• Fast-from sample to result in the same day

How It Works
The method relies on quantitative real-time PCR. First, a standard curve is created by serially diluting the included multi-animal DNA standard. Then assays for the target species DNA (from one of the kits listed above) and for all animal DNA (from the RapidFinder™ Quant Multi-Animal Set) are run side-by-side on both the food sample DNA and the DNA standards. This approach enables rapid and accurate quantification and reporting of the percentage of target animal species as compared to total animal in a sample. Included in each reaction mix is an internal positive control detected in the VIC™ dye channel, which is used to verify the absence of PCR inhibitors.

The RapidFinder™ Quant Multi-Animal Set is optimized and validated for use with the Applied Biosystems™ QuantStudio™ 5 Real‑Time PCR Instrument and the Applied Biosystems™ 7500 Fast Real‑Time PCR Instrument.

Sample Preparation
DNA sample preparation for the RapidFinder™ animal species identification and quantification workflow is accomplished with the easy-to-use GMO Extraction Kit (Cat. No. 4466336). Using 10 g of sample rather than the smaller sample sizes accommodated by most other DNA isolation procedures minimizes sampling errors. The GMO Extraction Kit is compatible with raw and processed meat, as well as composite food samples.

사양

검출 방법Primer-probe

PCR 방법qPCR

반응 속도Standard

용도(장비)Applied Biosystems™ QuantStudio™ 5 Real‑Time PCR Instrument and Applied Biosystems™ 7500 Fast Real‑Time PCR Instrument

제품라인RapidFinder™

수량48 reactions

종Multi-animal

용도(애플리케이션)Food and Beverage

반응 수48 reactions

샘플 종류Food & Environmental Samples

Unit SizeEach