Thermo Fisher Scientific HRI Polyclonal Antibody
Thermo Fisher Scientific의 HRI Polyclonal Antibody는 인간 EIF2AK1 단백질을 표적으로 하는 rabbit IgG 항체로, WB, IHC, ICC/IF에 적합합니다. 항원 친화 크로마토그래피로 정제되었으며, 액상 형태로 제공됩니다. 연구용으로만 사용 가능합니다.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.04–0.4 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:20–1:50 |
| Immunocytochemistry (ICC/IF) | 0.25–2 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human EIF2AK1. Recombinant protein control fragment (Product #RP-91431). |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2789709 |
Product Specific Information
Immunogen sequence:
PDPEYDESDV PAEIQVLKEP LQQPTFPFAV ANQLLLVSLL EHLSHVHEPN PLRSRQVFKL LCQTFIKMGL LSSFTCSDEF SSLRLHHNRA ITHLMRSAKE RVRQDPCEDI SRIQKIRSRE VALEAQTSRY LNEFEELAIL GKGGYGR
Target Information
EIF2AK1 (HRI) is one of four kinases that specifically phosphorylate Ser51 of translation initiation factor eIF2-alpha in response to various environmental stresses, leading to decreased protein synthesis. In the case of EIF2AK1, signaling is initiated by low levels of heme. The HRI gene is localized to 7p22, where its 3′ end slightly overlaps the 3′ end of the gene JTV1. The two genes are transcribed from opposite strands, and kinase activity is induced by low levels of heme and inhibited by the presence of heme.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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