
Thermo Fisher Scientific Sheep anti-Rat IgG (H+L) Secondary Antibody, FITC
Thermo Fisher Scientific의 Sheep anti-Rat IgG (H+L) FITC 결합 2차 항체는 Rat IgG 검출용으로 설계된 고순도 폴리클로날 항체입니다. ICC/IF, Flow Cytometry 등 다양한 응용에 적합하며, 498/517 nm 형광 특성을 가집니다. 연구용으로 높은 감도와 특이성을 제공합니다.
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Applications
Immunocytochemistry (ICC/IF)
- Tested Dilution: 1:1,000–1:5,000
Flow Cytometry (Flow)
- Tested Dilution: 1:500–1:2,500
Immunomicroscopy (IM)
- Tested Dilution: Assay-dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Rat |
| Host / Isotype | Sheep / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Rat whole molecule |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 2 mg/mL |
| Purification | IgG fraction |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl, 1% BSA |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Store in the dark. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10989428 |
| Target | IgG |
| Antibody Form | Whole Antibody |
Additional Formats
- AP
- Biotin
- HRP
- TRITC
- Custom conjugation available upon request
Product Specific Information
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Anti-Rat secondary antibodies are affinity-purified and show well-characterized specificity for rat immunoglobulins. They are useful for detection, sorting, or purification of rat IgG targets. Secondary antibodies enhance versatility and sensitivity by enabling multiple detection systems (HRP, AP, fluorescence) and signal amplification. These antibodies are typically produced by immunizing host animals with pooled rat immunoglobulins and purified via immunoaffinity chromatography or conjugation to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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