Thermo Fisher Scientific Lactate Dehydrogenase Polyclonal Antibody
Rabbit muscle 유래 LDH 단백질에 대한 goat polyclonal 항체로, WB, ICC/IF, ELISA, IP 등 다양한 면역실험에 사용 가능. 고농도(82 mg/mL) 동결건조 형태로 제공되며, 장기보관 시 -20°C에서 안정적. 대부분 포유류 LDH-A 및 LDH-B와 교차반응 가능.
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Thermo Fisher Scientific Lactate Dehydrogenase Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:200 |
| ELISA | 1:4,000–1:20,000 |
| Immunoprecipitation (IP) | 1:500–1:2,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Rabbit |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Whole goat serum immunized with full-length lactate dehydrogenase protein isolated from rabbit muscle |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 82 mg/mL |
| Storage Buffer | 0.02M potassium phosphate/whole serum, pH 7.2, with 0.15M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Store vial at 4°C prior to restoration.
- For extended storage, aliquot contents and freeze at -20°C or below.
- Avoid freeze/thaw cycles.
- Centrifuge if not completely clear after standing at room temperature.
- Stable for several weeks at 4°C as undiluted liquid.
- Dilute only prior to immediate use.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum, purified and partially purified Lactate Dehydrogenase (Rabbit Muscle).
BLAST analysis suggests cross-reactivity with muscle and heart isoforms (LDH-A and LDH-B) from most mammalian species.
Target Information
Lactate dehydrogenase (LDH) is a key enzyme in glycolysis that converts pyruvate to lactate while interconverting NADH and NAD+.
In mammals, LDH is encoded by three genes:
- LDHA (M or muscle form)
- LDHB (H or heart form)
- LDHC (X or testis form)
Human LDHs share 84–89% sequence similarity and 69–75% amino acid identity.
There are five LDH isoenzymes based on the M/H chain ratio in the tetrameric structure.
LDHA expression is hypoxia-inducible and regulated by HIF1α, while LDHB is upregulated in many cancers.
Mutations in LDHA can cause Glycogen Storage Disease 11 (GSD11).
For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.
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