Thermo Fisher Scientific DKK3 Polyclonal Antibody
DKK3 단백질을 인식하는 Thermo Fisher Scientific의 토끼 폴리클로날 항체. Western blot 및 면역세포염색에 사용 가능. 인간 및 생쥐 시료 반응성. 합성 펩타이드 면역원 사용, 액상 형태로 1 mg/mL 농도. 연구용으로만 사용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human DKK3 (Accession Q9UBP4), corresponding to amino acid residues E320–I350 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2850987 |
Product Specific Information
Antibody detects endogenous levels of total DKK3.
Target Information
DKK3, like DKK1, DKK2, and DKK4, possesses an N-terminal signal peptide and two conserved cysteine-rich domains separated by a linker region containing ten cysteine residues each.
The second cysteine region may have a lipid-binding function facilitating WNT/DKK interactions at the plasma membrane.
The linker region contains 50–55 amino acids in DKK1, DKK2, and DKK4, but only 12 amino acids in DKK3.
All DKKs have several potential sites for cleavage by furin-type proteases.
Northern blot analysis revealed wide expression of the DKK3 transcript, with highest expression in heart, brain, and spinal cord.
In situ hybridization shows highest expression in mouse brain, eye, and heart.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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