Thermo Fisher Scientific MDFI Polyclonal Antibody
MDFI 단백질을 인식하는 Rabbit Polyclonal 항체로, Western blot, IHC, ICC/IF에 사용 가능. 인간, 마우스, 랫트 반응성. 합성 펩타이드 면역원 기반으로 제작되었으며, 액상 형태로 -20°C 보관. 연구용 전용 제품.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human MDFI (Accession Q99750), corresponding to amino acid residues N102–T152 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2851117 |
Product Specific Information
This antibody detects endogenous levels of total MDFI protein.
Target Information
MDFI is a transcription factor that negatively regulates other myogenic family proteins. Studies of the mouse homolog (I-mf) indicate interference with myogenic factor function by masking nuclear localization signals and preventing DNA binding. Knockout mouse studies show defects in vertebrae and rib formation, involving cartilage development. MDFI also interferes with the DNA-binding activity of MyoD family members and plays an important role in trophoblast and chondrogenic differentiation.
For Research Use Only.
Not for use in diagnostic procedures or resale without express authorization.
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