Thermo Fisher Scientific NEEP21 Polyclonal Antibody
NEEP21 단백질을 인식하는 Rabbit Polyclonal 항체로, Western blot, IHC, ICC, ELISA 등에 사용 가능. 인간, 마우스, 랫드 등 다양한 종에 반응. Protein A로 정제된 액상 형태로, 안정적인 신호 검출에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | Assay-dependent |
| Immunohistochemistry (Frozen) (IHC-F) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1:500–1:1,000 |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human, Mouse, Non-human primate, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH-conjugated synthetic peptide derived from human NEEP21 (amino acids 1–100) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
NEEP21 (neuron-enriched endosomal 21kDa protein), also known as brain neuron cytoplasmic protein 1, NSG1 (neuron-specific proteins family member 1), P21, or D4S234E, is a single-pass type II membrane protein belonging to the NSG family. It is highly expressed during neuronal maturation but is downregulated in adult tissues. NEEP21 predominantly localizes to Rab4-positive early endosomes in the somatodendritic neuronal compartment and is essential for proper receptor sorting and recycling in neurons.
It associates with GRIP1 and GluR-2, mediating the surface expression of GluR-2. Disruption of this interaction results in GluR-2 accumulation in early endosomes, altering synaptic current properties. NEEP21 also forms a complex with the SNARE protein Syntaxin 13 (Syntaxin 12) and participates in the recycling of transferrin receptors (TFRs) and NTR2 (Neurotensin receptor 2).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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