Thermo Fisher Scientific IRF8 Polyclonal Antibody
IRF8 단백질을 인식하는 Rabbit Polyclonal Antibody로, WB, IHC, ICC/IF에 사용 가능. Human 시료 반응성. 항원 친화 크로마토그래피로 정제되었으며 PBS 버퍼에 40% 글리세롤 포함. 연구용으로만 사용.
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Thermo Fisher Scientific IRF8 Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.04–0.4 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 0.25–2 µg/mL |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human IRF8. Recombinant protein control fragment (Product #RP-101888) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2789171 |
Product Specific Information
Immunogen sequence:
QGVFVKRLCQ GRVFCSGNAV VCKGRPNKLE RDEVVQVFDT SQFFRELQQF YNSQGRLPDG RVVLCFGEEF PDMAPLRSKL ILVQIEQLYV RQLAEEAGKS CGAGSVMQAP EEPPPDQVFR MFPDICASHQ RSFFRENQQI T
Target Information
IRF8 (interferon regulatory factor 8) is a transcriptional activator or repressor that specifically binds to the upstream regulatory region of type I IFN and IFN-inducible MHC class I genes.
It plays a negative regulatory role in immune cells and is involved in CD8+ dendritic cell differentiation by forming a complex with the BATF-JUNB heterodimer. This complex recognizes the AICE sequence, an immune-specific regulatory element, leading to cooperative binding and gene activation.
Mutations in the IRF8 gene can result in immunodeficiency types 32A and 32B.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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