Thermo Fisher Scientific Phospho-NFkB p65 (Ser311) Polyclonal Antibody
NFkB p65 (Ser311) 인산화 부위를 인식하는 Thermo Fisher Scientific의 폴리클로날 항체로, 인간·마우스·랫트 시료에 반응합니다. WB, IHC, ICC/IF에 사용 가능하며, 고순도 친화 크로마토그래피 정제 및 안정적 PBS/glycerol 저장용액 제공. 연구용 전용 제품.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:100 |
| Immunocytochemistry (ICC/IF) | 1:100–1:200 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide sequence around phosphorylation site of serine 311 (F-K-S(p)-I-M) derived from Human NF-kappa-B-p65 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2554328 |
Product Specific Information
- Suggested positive control for Western blot: HeLa cells
- Suggested positive control for IHC: Human breast carcinoma
- Suggested positive control for ICC/IF: HeLa cells
Target Information
Nuclear factor kB p65 (NF-kB p65) is encoded by the RELA gene and located on chromosome 11 in humans. Also known as RelA, it belongs to the Rel family of proteins and forms a heterodimer with p50 or p52 subunits. Upon TNFα receptor binding, IKK phosphorylates IκB, leading to its proteasomal degradation. NF-kB p65 plays a key role in transcription of the immunoglobulin κ gene in mature B-lymphoid cells.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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