Thermo Fisher Scientific HA Tag Monoclonal Antibody (2-2.2.14)

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1 -0.2 µg/mL

View 129 publications 129 publications

Immunocytochemistry (ICC/IF)

2 µg/mL

View 21 publications 21 publications

Flow Cytometry (Flow)

-

View 7 publications 7 publications

Immunoprecipitation (IP)

5 µg

View 21 publications 21 publications

ChIP assay (ChIP)

-

View 6 publications 6 publications

in situ PLA (PLA)

-

View 2 publications 2 publications

RNA Immunoprecipitation (RIP)

-

View 1 publication 1 publication

In vitro Assay (IV)

-

View 2 publications 2 publications

Miscellaneous PubMed (Misc)

-

View 7 publications 7 publications

Product Specifications

Species Reactivity

Tag

Published species

Tag

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

2-2.2.14

Immunogen

HA peptide YPYDVPDYA derivitized to ovalbumin.

Conjugate

Unconjugated Unconjugated Unconjugated

View additional formats

• Alexa Fluor 488
• Alexa Fluor 555
• Alexa Fluor 647
• Biotin
• DyLight 488
• DyLight 550
• DyLight 650
• DyLight 680
• DyLight 800 4X PEG
• HRP
• Request custom conjugation

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.4

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10978021

Product Specific Information

26183 is specific for the HA peptide YPYDVPDYA. The antibody has been used successfully in Western blot and immunoprecipitation applications.

HA Synthetic Peptide (Product # 26184) is available for use in neutralization and control experiments and to competitively elute HA-tagged fusion proteins from immobilized anti-HA affinity resin.

Target Information

HA Tag (hemagglutinin tag) is an epitope tag that provides a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. HA (hemagglutinin) is a surface glycoprotein derived from influenza hemagglutinin, and is commonly expressed as a tag in conjunction with proteins to aid in identification and purification of the tagged protein for functional analysis. HA tag antibodies can be used to facilitate protein detection or co-immunopreciptation of tagged proteins.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.