Thermo Fisher Scientific HK2 Polyclonal Antibody
HK2 단백질을 인식하는 Rabbit Polyclonal 항체로, WB, IHC(P), ICC/IF에 사용 가능. Human, Mouse, Rat 반응성. Affinity chromatography로 정제된 액상 형태, -20°C 보관. 연구용으로 적합하며 Hexokinase II의 내인성 발현 검출에 유용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:100–1:400 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human HK2 (Accession P52789), corresponding to amino acid residues P884–R917 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2851737 |
Product Specific Information
Antibody detects endogenous levels of total Hexokinase II.
Target Information
In vertebrates, there are four major glucose-phosphorylating isoenzymes designated hexokinase I, II, III, and IV. Hexokinase activity is involved in the first step of several metabolic pathways, including phosphorylation of glucose to produce glucose-6-phosphate, committing glucose to the glycolytic pathway. Hexokinase is an allosteric enzyme inhibited by its product GLC-6-P. Hexokinase 2 is the predominant isozyme expressed in insulin-responsive tissues such as skeletal muscle. Its expression is insulin-responsive and is implicated in the elevated glycolysis rate in rapidly growing cancer cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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