Invitrogen™

pBAD-DEST49 Gateway™ Destination Vector

The pBAD-DEST49 Gateway™ destination vector is designed for rapid cloning with a Gateway™ entry clone using lambda phage site-specific recombination자세히 알아보기

The pBAD-DEST49 Gateway™ destination vector is designed for rapid cloning with a Gateway™ entry clone using lambda phage site-specific recombination and subsequent high-level, tightly regulated expression in E. coli. The pBAD-DEST49 vector features:

• The ara_BAD promoter for tightly regulated expression in _E. coli
• HP-thioredoxin fusion for improved protein yield and solubility
• Enterokinase cleavage site for efficient cleavage of the N-terminal fusion with EKMax™
• C-terminal V5 and 6xHis tags for efficient detection and purification of fusion proteins
• R sites for efficient recombination with any _att_L-flanked Gateway™ entry vector

사양

구성 또는 유도성 시스템Inducible

유도제Arabinose

프로모터araBAD

제품 유형Gateway System Destination Expression Vector

선택 제제(진핵)None

항생제 내성 박테리아Ampicillin (AmpR)

분열EK (Enterokinase) Recognition Site

단백질 태그His Tag (6x), V5 Epitope Tag, Thioredoxin

클로닝 방법Gateway

수량6 μg

벡터pBAD, pDEST

제품라인Gateway™

Unit SizeEach