Thermo Fisher Scientific Bcl-W Monoclonal Antibody (4G12D11)
인간 Bcl-W 단백질을 인식하는 Thermo Fisher Scientific의 마우스 단클론 항체입니다. Western blot, ELISA, 유세포분석에 사용 가능하며, 고순도 Protein G 정제 및 PBS 버퍼에 보존됩니다. 연구용으로만 사용됩니다.
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Applications
| Application | Tested Dilution | Notes |
|---|---|---|
| Western Blot (WB) | 1:500–1:2,000 | |
| Flow Cytometry (Flow) | Assay-dependent | |
| ELISA | 1:10,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 4G12D11 |
| Immunogen | Purified recombinant fragment of human BCL2L2 (AA: 6–118) expressed in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_3091017 |
Target Information
Using a PCR-based strategy, an additional protein with life-promoting activity, designated Bcl-w, has been identified. The protein is highly conserved between mouse and human and is encoded by a gene located near the TCRα gene on chromosome 14.
Bcl-w is expressed in myeloid cell lines but not in T and B lymphocytes, and can be found in a wide range of tissues.
An alternative splicing event in exon 4 results in two transcripts:
- Bcl-w: encodes a protein of 193 amino acids
- Bcl-w/rox: encodes a protein 333 amino acids in length
The rox portion of Bcl-w/rox shows a striking 66% amino acid sequence identity with the Drosophila rox2 protein; however, the Bcl-w/rox transcript may be expressed at very low levels.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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