Thermo Fisher Scientific DCP1A Recombinant Rabbit Monoclonal Antibody (ARC1421)
Thermo Fisher Scientific의 DCP1A 재조합 Rabbit Monoclonal Antibody (ARC1421)는 인간 및 마우스 반응성을 가지며 WB, IHC, ICC/IF, ELISA에 적합합니다. HEK293 세포 발현 시스템을 사용한 고순도 항체로, 안정적인 액상 형태로 제공되어 연구용으로 최적화되어 있습니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | ARC1421 |
| Immunogen | Synthetic peptide corresponding to amino acids 483–582 of human DCP1A (Q9NPI6) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol, 0.05% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | –20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2849596 |
Product Specific Information
Immunogen sequence:
PVAGAPLVTA TTTAVSSVLL APSVFQQTVT RSSDLERKAS SPSPLTIGTP ESQRKPSIIL SKSQLQDTLI HLIKNDSSFL STLHEVYLQV LTKNKDNHNL
Target Information
Signal transduction of TGF-beta superfamily members is regulated by Smad proteins. Smads relay signals from the cell membrane to the nucleus, influencing specific gene transcription. Smad4 plays an essential role in TGF-beta-induced transcriptional activation, where phosphorylated receptor-associated Smads associate with Smad4.
SMIF (Smad4-interacting protein) and Smad4 form complexes with TGF-beta and BMP4, facilitating nuclear translocation. The SMIF-Smad4 interaction occurs via an EVH1/WH1 domain on SMIF and a proline-rich activation domain on Smad4. Deletion of the Smad4-interacting domain in SMIF abolishes TGF-beta-induced nuclear translocation. The human SMIF gene encodes a 70 kDa protein expressed ubiquitously.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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