Thermo Fisher Scientific Proteasome 20S C2 Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: Assay-dependent

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: Assay-dependent

Product Specifications

Target Information

Proteolytic degradation is essential for maintaining proper levels of short-lived and regulatory proteins involved in metabolism, stress response, antigen presentation, receptor modulation, cell cycle regulation, transcription, and signaling.
The ubiquitin-proteasome pathway is responsible for degrading most cytosolic and nuclear proteins in eukaryotic cells, while others are degraded via the vacuolar pathway involving endosomes, lysosomes, and the endoplasmic reticulum.

The 26S proteasome is an ATP-dependent, multisubunit (~31 subunits) molecular complex with a molecular weight of approximately 2.5 MDa. It consists of a 20S proteolytic core complex capped by one or two 19S regulatory subunit complexes. The 19S subunits recognize ubiquitinated proteins and facilitate their unfolding and translocation into the 20S core for degradation.

An enzymatic cascade mediates the attachment of multiple ubiquitin molecules to lysine residues on target proteins. Defects in the ubiquitin-proteasome pathway are associated with several genetic diseases, including cystic fibrosis, Angelman’s syndrome, and Liddle syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.