Thermo Fisher Scientific Human STK17B (DRAK2), GST Tag Recombinant Protein

Applications

• Kinase Assay (KA)

Tested Dilution: Assay-dependent

Product Specifications

Product Specific Information

• For maximum recovery, spin prior to use.
• Aliquots of 5 µg, 10 µg, and 20 µg sizes are not recommended; use original packaging until exhausted.
• For larger sizes, reduce freeze/thaw cycles by preparing aliquots (do not prepare aliquots below 20 µL).
• Do not store diluted kinase.
• Guaranteed for 6 months from date of purchase if properly stored at -80°C.

Recombinant full-length human STK17B was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Features:

• Validated in LanthaScreen™ Kinase Binding Assay
• Protocols available for simple use and optimized performance
• Multiple pack sizes available for convenient use

Related Product:
LanthaScreen™ Eu Kinase Binding Assay

Protein Form: Recombinant

Target Information

STK17B (also known as DRAK2) is a member of the serine/threonine kinase family related to death-associated protein kinase that triggers apoptosis. It is selectively important for T-cell survival, and inhibition of STK17B has therapeutic potential for autoimmune diseases. T-cell survival depends on balanced receptor and co-stimulatory signals; deficiency of STK17B can affect autoimmune susceptibility without general immune suppression.

Protein kinases are key in signal transduction responding to external stimuli. Several kinases, including ZIP kinase, DRAK1, and DRAK2, are associated with apoptosis. Overexpression of these kinases in NIH 3T3 cells induces apoptotic morphological changes. The kinase domains of DRAKs and ZIP kinase are homologous to DAP kinase, which is involved in interferon-γ–induced apoptotic signaling. These findings suggest that the C-terminal domain of DRAKs is crucial for apoptosis induction.

For Research Use Only.
Not for use in diagnostic procedures or resale without express authorization.