Thermo Fisher Scientific FIR Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

0.04-0.4 µg/mL

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein corresponding to Human FIR. Recombinant protein control fragment (Product #RP-95947). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:FIR,uniProtId:O94887-1,ncbiNodeId:9606,antigenRange:3-79,antigenLength:1054,antigenImageFileName:PA5-56681_FIR_O94887-1_Rabbit.svg,antigenImageFileNamePDP:PA5-56681_FIR_O94887-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2641644

Product Specific Information

Immunogen sequence: EIEGTYRVLQ TAGMRLGAQT PVGVSTLEPG QTLLPRMQEK HLHLRVKLLD NTMEIFDIEP KCDGQVLLTQ VWKRLNL

Highest antigen sequence identity to the following orthologs: Mouse - 78%, Rat - 79%.

Target Information

FARP2 (FERM, RhoGEF and pleckstrin domain-containing protein 2), also known as PLEKHC3 or FERM domain including RhoGEF (FIR), is a 1,545 amino acid protein that contains one FERM domain, one DH domain and two PH domains. It exists as two alternatively spliced isoforms that are abundantly expressed in brain, lung, and testis as well as in embryonic hippocampal and cortical neurons. FARP2 functions as a Rho-guanine nucleotide exchange factor that activates RAC1 and is thought to regulate neurite remodeling of embryonic neurons. Sema3A binding to neuropilin-1 induces the dissociation of FARP2 from plexin-A1, thereby activating FARP2`s Rac GEF activity which is critical for repulsion of outgrowing axons and suppression of neuronal adhesion. Downregulation of the FARP2 gene has been implicated in autism.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.