Takara MseI (RspRSII) restriction enzyme
MseI(RspRSII)는 5'-TTAA-3' 인식서열을 절단하는 제한효소로, 60°C에서 최적 활성을 보이며 DNA 메틸화에 민감하지 않습니다. 고농도 글리세롤에서 별자리 활성(star activity)을 보일 수 있습니다. 게놈 DNA 절단 및 메틸화 DNA 분석 전처리에 적합합니다.
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MseI (RspRSII) Restriction Enzyme
Recognition Site
T | TAA
A ATT |Buffer
- Supplied buffer: T + BSA
DNA Methylation Sensitivity
- Not sensitive to DNA methylation
Reaction Conditions
- Optimal reaction temperature: 60°C
Product Specifications
| 항목 | 내용 |
|---|---|
| Source | Escherichia coli carrying a plasmid encoding the RspRSII gene |
| Concentration | 10 units/µl |
| Substrate for unit definition | Lambda DNA |
| Star activity | Cleavage at alternative site(s) occurs in the presence of high concentrations of glycerol |
| Inactivation | Residual activity observed after EtOH precipitation or incubation at 70°C for 15 minutes. No residual activity observed after phenol treatment |
| Recognition sequence | 5'-TTAA-3' |
| Optimal reaction temperature | 60°C |
| Quality testing | Overdigestion, genome DNA analysis, ligation-recutting, and pKF3 cloning |
Description
MseI, an isoschizomer of RspRSII, is a restriction enzyme that cleaves within the recognition sequence 5'-TTAA-3'.
It is exceptionally stable and can be used to pretreat (fragment) genomic DNA prior to enrichment of methylated DNA using the EpiXplore Methylated DNA Enrichment Kit.
Additional Product Information
Please refer to the product’s Certificate of Analysis for details on storage conditions, product components, and technical specifications.
Certificates of Analysis and Kit Components Lists are available under the Documents tab.
Takara Bio USA, Inc.
United States/Canada: +1.800.662.2566
Asia Pacific: +1.650.919.7300
Europe: +33.(0)1.3904.6880
Japan: +81.(0)77.565.6999
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.
© 2023 Takara Bio Inc. All Rights Reserved.
All trademarks are the property of Takara Bio Inc. or its affiliates.
Additional product, intellectual property, and restricted use information available at takarabio.com.
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