Thermo Fisher Scientific FITC/5-FAM/6-FAM Recombinant Rabbit Monoclonal Antibody (4L8R5)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:2,000-1:4,800

-

ELISA (ELISA)

1:500-1:2,000

-

Product Specifications

Species Reactivity

Chemical

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

4L8R5

Immunogen

FITC

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.3, with 50% glycerol, 0.05% BSA

Contains

0.09% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Product Specific Information

Immunogen Sequence: FITC conjugated to KLH

Target Information

FITC (Fluorescein Isothiocyanate), 5-FAM (5-Carboxyfluorescein), and 6-FAM (6-Carboxyfluorescein) are commonly used gene targets for fluorescent labeling in molecular biology research. These dyes belong to the fluorescein family and emit a green fluorescence when excited by specific wavelengths of light. FITC, 5-FAM, and 6-FAM are utilized in various techniques such as fluorescence in situ hybridization (FISH), flow cytometry, immunofluorescence, and DNA sequencing. By attaching these dyes to gene-specific probes or primers, researchers can visualize and detect gene expression levels, identify specific DNA or RNA sequences, and study cellular processes. The compatibility of FITC, 5-FAM, and 6-FAM with different imaging systems and their bright green fluorescence make them valuable tools in molecular biology, aiding in the understanding of gene function and cellular dynamics.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.