Takara LA PCR in vitro Cloning Kit

The LA PCR in vitro Cloning Kit is designed specifically for long-range PCR and cloning of plasmid, cDNA, or genomic DNA regions that contain a known sequence flanking one end. This cloning kit for long fragments uses the Takara LA Taq enzyme for longer amplification and improved fidelity. Amplification is accomplished via cassette-mediated PCR whereby long DNA fragments, such as those found in genomic DNA, are used without the need for time-consuming library construction and screening. Users will need to design and prepare primers corresponding to the known region of the DNA to be amplified.

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Applications

• Long-fragment cloning
• Long-range PCR and cloning

Product citations

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Isegawa, Y. et al. Selective amplification of cDNA sequence from total RNA by cassette-ligation mediated polymerase chain reaction (PCR): application to sequencing 6.5 kb genome segment of hantavirus strain B-1. Mol. Cell. Probes 6, 467–75 (1992).

Jansen, W. T. M. et al. Novel mobile variants of staphylococcal cassette chromosome mec in Staphylococcus aureus. Antimicrob. Agents Chemother. 50, 2072–8 (2006).

Li, H.-Y. et al. Colonization by the arbuscular mycorrhizal fungus Glomus versiforme induces a defense response against the root-knot nematode Meloidogyne incognita in the grapevine (Vitis amurensis Rupr.), which includes transcriptional activation of the class III chitinase gene VCH3. Plant Cell Physiol. 47, 154–63 (2006).

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Tam, W. et al. Mutational analysis of PRDM1 indicates a tumor-suppressor role in diffuse large B-cell lymphomas. Blood 107, 4090–100 (2006).

Additional product information

Please see the product`s Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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