Takara LA PCR in vitro Cloning Kit

Takara LA PCR in vitro Cloning Kit

The LA PCR in vitro Cloning Kit is designed specifically for long-range PCR and cloning of plasmid, cDNA, or genomic DNA regions that contain a known sequence flanking one end.
This cloning kit for long fragments uses the Takara LA Taq enzyme for longer amplification and improved fidelity. Amplification is accomplished via cassette-mediated PCR, allowing the use of long DNA fragments (such as genomic DNA) without the need for time-consuming library construction and screening.
Users must design and prepare primers corresponding to the known region of the DNA to be amplified.

Applications

• Long-fragment cloning
• Long-range PCR and cloning

Product Citations

• Deeraksa, A. et al. Characterization and spontaneous mutation of a novel gene, polE, involved in pellicle formation in Acetobacter tropicalis SKU1100. Microbiology 151, 4111–20 (2005).
• Hayashi, M., Ishibashi, T., Tanaka, K. & Kasahara, M. The mouse genes encoding the third pair of beta-type proteasome subunits regulated reciprocally by IFN-gamma: structural comparison, chromosomal localization, and analysis of the promoter. J. Immunol. 159, 2760–70 (1997).
• Isegawa, Y. et al. Selective amplification of cDNA sequence from total RNA by cassette-ligation mediated PCR: application to sequencing 6.5 kb genome segment of hantavirus strain B-1. Mol. Cell. Probes 6, 467–75 (1992).
• Jansen, W. T. M. et al. Novel mobile variants of staphylococcal cassette chromosome mec in Staphylococcus aureus. Antimicrob. Agents Chemother. 50, 2072–8 (2006).
• Li, H.-Y. et al. Colonization by the arbuscular mycorrhizal fungus Glomus versiforme induces a defense response against the root-knot nematode Meloidogyne incognita in grapevine (Vitis amurensis Rupr.), which includes transcriptional activation of the class III chitinase gene VCH3. Plant Cell Physiol. 47, 154–63 (2006).
• Sonke, T. et al. L-selective amidase with extremely broad substrate specificity from Ochrobactrum anthropi NCIMB 40321. Appl. Environ. Microbiol. 71, 7961–73 (2005).
• Tam, W. et al. Mutational analysis of PRDM1 indicates a tumor-suppressor role in diffuse large B-cell lymphomas. Blood 107, 4090–100 (2006).

Additional Product Information

Please refer to the product’s Certificate of Analysis for details on storage conditions, product components, and technical specifications.
The Kit Components List provides information on included reagents and materials.
Both documents are available under the Documents tab on the product page.

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