Thermo Fisher Scientific CYP2E1 Monoclonal Antibody (OTI5F11), TrueMAB
CYP2E1 단백질을 인식하는 mouse monoclonal antibody로, Western blot, IHC, ICC/IF에 사용 가능. 인간 시료에 반응하며, carrier-free 형태로 제공되어 conjugation 실험에 적합. 약물대사 및 지방합성 관련 연구에 활용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50 |
| Immunocytochemistry (ICC/IF) | 1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI5F11 |
| Immunogen | Full length human recombinant CYP2E1 protein produced in HEK293T cells |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
CYP2E1 encodes a cytochrome P450 enzyme involved in drug metabolism and synthesis of cholesterol, steroids, and other lipids.
This enzyme localizes to the endoplasmic reticulum and is induced by ethanol, diabetic state, and starvation.
It metabolizes endogenous substrates (ethanol, acetone, acetal) and exogenous substrates (benzene, carbon tetrachloride, ethylene glycol, nitrosamines).
CYP2E1 activity is associated with processes such as gluconeogenesis, hepatic cirrhosis, diabetes, and cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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