Thermo Fisher Scientific Rabbit anti-Goat IgG (Heavy Chain) Superclonal Secondary Antibody, Alexa Fluor 647
Rabbit에서 생산된 Goat IgG(Heavy Chain) 특이적 Superclonal 2차 항체로, Alexa Fluor 647로 표지되어 있습니다. Western blot 및 면역세포염색에 적합하며, 높은 특이성과 재현성을 제공합니다. 최소한의 교차 반응성과 일관된 로트 간 품질을 보장합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,500–1:10,000 |
| Immunocytochemistry (ICC/IF) | 0.1–1 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Goat |
| Host / Isotype | Rabbit / IgG |
| Expression System | Expi293 |
| Class | Recombinant Superclonal |
| Type | Secondary Antibody |
| Immunogen | Recombinant full-length protein |
| Conjugate | Alexa Fluor™ 647 |
| Excitation / Emission Max | 650 / 671 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Gravity column chromatography |
| Storage Buffer | PBS |
| Contains | 5 mM sodium azide |
| Storage Conditions | 4°C, store in dark, Do not freeze |
| Shipping Conditions | Wet ice |
| RRID | AB_2536082 |
| Target | IgG (Heavy chain) |
| Antibody Form | Whole Antibody |
Available Conjugate Formats
- Alexa Fluor 488
- Alexa Fluor 555
- Alexa Fluor 680
- Alexa Fluor 790
- HRP
- Custom conjugation available upon request
Product Specific Information
Each lot is validated for sensitivity and specificity using ELISA.
Minimal cross-reactivity with mouse, rat, human, bovine, guinea pig, and donkey IgG is observed.
Recombinant rabbit polyclonal (Superclonal) antibodies combine the advantages of monoclonal and polyclonal antibodies — offering high sensitivity, specificity, and reproducibility.
These antibodies contain a defined mixture of recombinant monoclonal antibodies that recognize multiple epitopes on the target, ensuring consistent performance across lots and eliminating biological variability typical of conventional polyclonal production.
Target Information
Anti-Goat secondary antibodies are affinity-purified and exhibit high specificity for goat immunoglobulins.
They are used for detection, sorting, or purification of goat antibodies and enable flexible detection systems such as HRP, AP, or fluorescence.
Signal amplification is achieved as multiple secondary antibodies can bind to a single primary antibody.
Production typically involves immunizing the host with pooled target immunoglobulins, followed by purification and optional modifications (e.g., immunoaffinity purification, fragmentation, conjugation) to enhance specificity and performance.
For Research Use Only. Not for use in diagnostic procedures.
Not for resale without express authorization.
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