Thermo Fisher Scientific HLA-DR Monoclonal Antibody (37.68), FITC

Applications

• Flow Cytometry (Flow)
  Tested Dilution: 1:5–1:10

Product Specifications

Product Specific Information

For FACS analysis, use 10 µL of the suggested working dilution to label 1×10⁶ cells in 100 µL.

Target Information

HLA-DR, like other MHC class II molecules, is a transmembrane glycoprotein composed of a 36 kDa alpha chain (DRA) and a 27 kDa beta chain (DRB).
The alpha chain gene contains 5 exons:

• Exon 1 encodes the leader peptide
• Exons 2 and 3 encode the extracellular domains
• Exon 4 encodes the transmembrane domain and cytoplasmic tail

DRA acts as the sole alpha chain for DRB1, DRB3, DRB4, and DRB5. The beta chain contains polymorphisms specifying peptide-binding specificities. Hundreds of DRB1 alleles have been described, and typing for these polymorphisms is routinely performed for bone marrow and kidney transplantation.

HLA-DR is expressed primarily on antigen-presenting cells such as:

• B lymphocytes
• Monocytes
• Macrophages
• Thymic epithelial cells
• Activated T lymphocytes

Three loci (DR, DQ, and DP) encode the major expressed products of the human class II region.
Human MHC class II molecules bind intracellularly processed peptides, present them to T-helper cells, and play a critical role in initiating the immune response.

HLA and MHC antibodies are essential in Immunopeptidomics, aiding in the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem mass spectrometry.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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