Thermo Fisher Scientific POLE2 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

-

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

0.25-2 µg/mL

-

Product Specifications

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.20 mg/mL

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2645746

Product Specific Information

Immunogen sequence: NAVEKQPLSS NMIERSVVEA AVQECSQSVD ETIEHVFNII GAFDIPRFVY NSERKKFLPL LMTNHPAPNL FGTPRDKAEM FRERYTILHQ RTHRHELFTP PVIGSHPDES GSKFQLKTIE TLLGSTTKIG DAIVLG

Highest antigen sequence identity to the following orthologs: Mouse - 89%, Rat - 90%.

Target Information

DNA replication, recombination and repair, all of which are necessary for genome stability, require the presence of exonucleases. In DNA replication, these enzymes are involved in the processing of Okazaki fragments, whereas in DNA repair, they function to excise damaged DNA fragments and correct recombinational mismatches. Exonucleases involved in these processes include DNA polymerases, including DNA pol delta and epsilon. DNA pol delta consists of two subunits-p125 which interacts directly with the sliding DNA clamp protein PCNA, and p50. DNA pol delta can be regulated by cell cycle proteins. DNA pol epsilon is a multiple subunit enzyme, the catalytic subunit of which is encoded by the POL2 gene. The exact reactions catalyzed by DNA pol delta and epsilon on leading and lagging strands have not yet been elucidated.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.