Thermo Fisher Scientific Peroxidase Polyclonal Antibody
Western blot, IHC, ELISA 등 다양한 면역분석에 사용 가능한 퍼옥시다아제 폴리클로날 항체. 토끼 유래 IgG로 제작되었으며, 높은 민감도와 안정성을 제공. 호스래디시 퍼옥시다아제로부터 유래한 항원 사용. 연구용으로만 사용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:1,000–1:5,000 |
| ELISA | 1:20,000–1:100,000 |
| Immunoprecipitation (IP) | 1:2,000–1:10,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Chemical |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Anti-Peroxidase antibody produced by repeated immunization of rabbits with peroxidase isolated from horseradish |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 90 mg/mL |
| Storage Buffer | Whole serum, PBS |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Store vial at 4°C prior to restoration.
- For extended storage, aliquot contents and freeze at -20°C or below.
- Avoid cycles of freezing and thawing.
- Centrifuge product if not completely clear after standing at room temperature.
- Stable for several weeks at 4°C as an undiluted liquid.
- Dilute only prior to immediate use.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum, purified and partially purified Peroxidase (Horseradish).
Cross-reactivity against peroxidase from other tissues and species may occur but has not been specifically determined.
Target Information
Horseradish peroxidase (HRP) is an enzyme commonly used in biochemistry and molecular biology research.
It catalyzes oxidation of various substrates in the presence of hydrogen peroxide.
HRP is widely used as a detection enzyme in ELISA and Western blot assays due to its high sensitivity and stability.
It can also serve as a tracer in enzyme-linked assays and as a labeling agent in fluorescence microscopy.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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