Thermo Fisher Scientific GeneChip™ HT HG-U133+PM Array Plate

GeneChip™ HT HG-U133+PM Array Plate

Description

Proven performance from the industry standard
The GeneChip™ HT HG-U133+ PM 96-Array Plate enables high-throughput expression profiling of multiple samples simultaneously, using the same content as the industry-standard GeneChip Human Genome U133 Plus 2.0 Array*.

Features and Capabilities

• Measure gene expression of more than 47,000 transcripts and variants, including over 33,000 well-characterized genes and UniGene clusters per sample
• Obtain accurate and reproducible data through multiple independent measurements for each transcript

Scatter plots represent median MAQC B - MAQC A signals for each array type. The high correlation coefficients indicate similar biological patterns are being observed (Fig. 1 and Fig. 2).

Key Benefits

• Increased productivity and efficiency through parallel processing
  • Process 16, 24, or 96 samples on a single array plate
• Same performance and content as the Human Genome U133 Plus 2.0 Array (cartridge format)
  • Strong signal and fold-change correlation to previous plate and cartridge designs
• Multiple independent measurements per transcript for higher confidence
  • Interrogates more sequence than a single probe measurement
• Convenient packaging
  • Available as individual 16-, 24-, and 96-array plates
  • Includes all necessary processing trays

Content Profile

Sequences used in the design of the arrays were selected from GenBank™, dbEST, and RefSeq. The majority of sequence clusters were created from the UniGene database (Build 133, April 20, 2001) and refined by comparison with other public databases, including:

• Washington University EST trace repository
• University of California, Santa Cruz Golden-Path human genome database (April 2001 release)

An additional set of sequence clusters was created from UniGene Build 159 (January 25, 2003) and refined by analysis and comparison with other databases, including:

• Washington University EST trace repository
• NCBI human genome assembly (Build 31)

Sequences were further analyzed for correct orientation, false priming, false clustering, alternative splicing, and alternative polyadenylation.

Design Improvements

*Two design changes were introduced with the GeneChip HT HG-U133+ PM Array Plate:

• Only perfect match (PM) probes from the cartridge design were retained, while mismatch (MM) probes were removed.
• Empirical data was used to select the best-performing probes, reducing the number of PM probes:
  • 42,461 probe sets were reduced from 11 to 9 probes
  • 6 probe sets were reduced from 11 to 10 probes

Specifications

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