Thermo Fisher Scientific DNA polymerase alpha Polyclonal Antibody
Thermo Fisher Scientific의 DNA polymerase alpha 폴리클로날 항체는 인간, 마우스, 랫트에 반응하며 다양한 면역실험(WB, IHC, ICC, ELISA)에 사용 가능. KLH 결합 합성 펩타이드 항원으로 제작되었으며, 고순도 Protein A 정제. 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | Assay-dependent |
| Immunohistochemistry (Frozen) (IHC-F) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1:500–1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH conjugated synthetic peptide derived from human DPOLA/DNA polymerase alpha (amino acids 1231–1350) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 1% BSA, 50% glycerol |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | −20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
DNA replication, recombination, and repair are essential for genomic stability and require exonucleases. During DNA replication, these enzymes process Okazaki fragments, while in DNA repair they excise damaged DNA and correct mismatches. The DNA polymerase family—including alpha, beta, delta, and epsilon—participates in these processes.
DNA pol delta and epsilon are multisubunit enzymes; delta consists of p125 (interacts with PCNA) and p50 subunits.
DNA pol gamma is responsible for mitochondrial DNA replication.
DNA pol zeta mediates damage-induced mutagenesis across tissues, and DNA pol theta functions as a DNA polymerase-helicase involved in interstrand crosslink repair.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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