Thermo Fisher Scientific p130Cas Polyclonal Antibody
Thermo Fisher Scientific의 p130Cas Polyclonal Antibody는 마우스 및 랫트 시료에서 p130Cas 단백질 검출에 적합한 Rabbit IgG 항체입니다. Western blot, IHC(F/P) 등 다양한 응용에 사용 가능하며, 항원 친화 크로마토그래피로 정제되었습니다. 장기 보관 시 -20°C에서 보관 권장.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:3,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:100–1:1,000 |
| Immunohistochemistry (Frozen) (IHC-F) | 1:100–1:1,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of mouse p130Cas. The exact sequence is proprietary. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 20% glycerol, 1% BSA |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2736287 |
Product Specific Information
- Positive Control: Mouse brain, rat brain, rat testis
- Predicted Reactivity: Mouse (100%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
The p130Cas (Crk-associated substrate) is a common cellular target of phosphorylation signaling via v-Crk and v-Src oncoproteins.
It has a unique structure containing an SH3 domain followed by multiple YXXP motifs and a proline-rich region.
p130Cas is involved in various biological processes including cell adhesion, migration, growth factor stimulation, cytokine receptor engagement, and bacterial infection.
Physiological roles include cardiovascular development, actin filament assembly, and Src-induced cell transformation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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