Thermo Fisher Scientific Phospho-Bcl-2 (Ser70) Monoclonal Antibody (R.65.1)
인산화된 Bcl-2 (Ser70)을 특이적으로 인식하는 Rabbit monoclonal 항체로, Western blot, ICC/IF, Flow cytometry에 적합합니다. 비인산화 Bcl-2 및 다른 Bcl-2 family 단백질과 교차반응하지 않으며, apoptosis 연구에 유용합니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000 | View 2 publications |
| Immunocytochemistry (ICC/IF) | 1:100 | - |
| Flow Cytometry (Flow) | 1:100 | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Monoclonal |
| Type | Antibody |
| Clone | R.65.1 |
| Immunogen | Synthetic phosphopeptide corresponding to residues surrounding serine 70 of human Bcl-2 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 57 µg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.01M HEPES, pH 7.5, with 0.15M NaCl, 100 µg/mL BSA, 50% glycerol |
| Contains | <0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_10978690 |
Product Specific Information
- It is not recommended to aliquot this antibody.
- This antibody is not cross-reactive with nonphosphorylated Bcl-2 at endogenous levels or with other Bcl-2 family members.
Target Information
BCL-2 is a key regulator of apoptosis that functions to either inhibit or promote cell death. Members of the BCL-2 family can form dimers to modulate apoptosis. For example, Bag1 forms a heterodimer with BCL-2, enhancing its anti-apoptotic effect. Bax and Bak are critical for cytochrome c release from mitochondria, initiating apoptosis.
Constitutive expression of BCL-2, such as through translocation to the Ig heavy chain locus, is associated with follicular lymphoma. In most follicular lymphomas, neoplastic germinal centers express high levels of BCL-2 alpha protein, whereas normal germinal centers do not. Two transcript variants of BCL-2 differ in their C-terminal ends. Overexpression of BCL-2 has been linked to cancers including B-cell lymphoma and prostate cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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