Thermo Fisher Scientific hnRNP M3/4 Monoclonal Antibody (2A6-2H3)

Applications

Western Blot (WB)

• Tested Dilution: 1:1,000

Immunoprecipitation (IP)

• Tested Dilution: 20 µL/1 mg lysate

Product Specifications

Product Specific Information

The recommended shelf life for this product is 1 year from date of receipt.

Target Information

Proteins that directly bind to nascent RNA polymerase II transcripts, the heterogeneous nuclear ribonucleoproteins (hnRNPs), play an important role in transcript-specific packaging and alternative splicing of pre-mRNAs.
A group of abundant hnRNPs, the M1–M4 proteins, appear as a cluster of four proteins. These M proteins are pre-mRNA binding proteins in vivo and bind strongly to poly(G) and poly(U) RNA homopolymers in vitro.
They belong to the ribonucleoprotein consensus sequence family of RNA-binding proteins, showing greatest similarity to a hypothetical RNA-binding protein from Saccharomyces cerevisiae.
The M proteins contain a hexapeptide-repeat region rich in methionine and arginine residues (MR repeat motif), resembling a repeat in the 64 kDa subunit of cleavage stimulation factor, which is involved in 3′-end maturation of pre-mRNAs.
Proteins immunologically related to M exist in divergent eukaryotes ranging from human to yeast.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.