Thermo Fisher Scientific hnRNP M3/4 Monoclonal Antibody (2A6-2H3)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

-

Immunoprecipitation (IP)

20 µL/1 mg lysate

-

Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

2A6-2H3

Immunogen

hnRNP M1-4 proteins

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

40 µg/mL

Purification

Affinity chromatography

Storage buffer

TBS, pH 7.0 to 8.0, with 0.1% BSA

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Wet ice

Product Specific Information

The recommended shelf life for this product is 1 year from date of receipt.

Target Information

Proteins that directly bind to nascent RNA polymerase II transcripts, the heterogenous nuclear ribonucleoproteins (hnRNPs), play an important role in both transcript-specific packaging and alternative splicing of pre-mRNAs. A group of abundant hnRNPs, the M1-M4 proteins, appear as a cluster of four proteins. The M proteins are pre-mRNA binding proteins in vivo, and they bind avidly to poly (G) and poly (U) RNA homopolymers in vitro. M proteins are members of the ribonucleoprotein consensus sequence family of RNA-binding proteins with greatest similarity to a hypothetical RNA-binding protein from Saccharomyces cerevisiae. The M proteins also possess an unusual hexapeptide-repeat region rich in methionine and arginine residues (MR repeat motif) that resembles a repeat in the 64kDa subunit of cleavage stimulation factor, which is involved in 3`-end maturation of pre-mRNAs. Proteins immunologically related to M exist in divergent eukaryotes ranging from human to yeast.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.