Thermo Fisher Scientific I kappa B-beta Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:2,000–1:10,000

Immunoprecipitation (IP)

• Tested Dilution: 2–5 µg/mg lysate

Product Specifications

Product Specific Information

• Recommended shelf life: 1 year from date of receipt.

Target Information

The NF-κB/Rel transcription factors are present in the cytosol in an inactive state, complexed with inhibitory IκB proteins. Activation of NF-κB typically occurs through phosphorylation-induced, proteasome-mediated degradation of IκB. This process involves activation of a high molecular weight IκB kinase (IKK) complex composed of three subunits: IKKα, IKKβ, and IKKγ. IKKα and IKKβ serve as catalytic subunits, while IKKγ acts as a regulatory subunit. Activation of IKK requires phosphorylation at Ser177 and Ser181 in IKKβ (Ser176 and Ser180 in IKKα), leading to conformational changes and kinase activation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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