Thermo Fisher Scientific Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 680
Alexa Fluor 680 형광이 결합된 Donkey anti-Mouse IgG (H+L) 항체로, Western blot 및 면역형광에 적합. 다양한 종에 대해 고도로 교차흡착되어 비특이적 결합 최소화. 근적외선 검출에 최적화된 고감도 형광 신호 제공. 연구용으로만 사용.
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Thermo Fisher Scientific Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 680
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:5,000–1:50,000 |
| Immunocytochemistry (ICC/IF) | 1:200–1:2,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Mouse |
| Host / Isotype | Donkey / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Gamma Immunoglobulin |
| Conjugate | Alexa Fluor™ 680 |
| Excitation / Emission Max | 681 / 704 nm |
| Form | Liquid |
| Concentration | 2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.5 |
| Contains | 5 mM sodium azide |
| Storage Conditions | 4°C, store in dark |
| Shipping Conditions | Ambient |
| RRID | AB_11180593 |
| Target | IgG |
| Cross Adsorption | Against bovine, chicken, goat, guinea pig, hamster, horse, human, rabbit, rat, and sheep serum |
| Antibody Form | Whole Antibody |
Product Specific Information
This secondary antibody is designed for fluorescent Western blot detection on various near-infrared fluorescence instruments. It enables multi-color and multiplexing detection when used with other antibodies conjugated to compatible Alexa Fluor™ dyes.
It is also suitable for immunofluorescent and fluorescent imaging applications with appropriate excitation and detection capabilities.
This antibody shows minimal cross-reactivity with bovine, chicken, goat, guinea pig, hamster, horse, human, rabbit, rat, and sheep serum proteins.
Product will be shipped at room temperature.
Target Information
Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins. They are useful in detection, sorting, or purification of mouse targets.
Secondary antibodies offer increased versatility and sensitivity through signal amplification, as multiple secondary antibodies can bind to a single primary antibody.
They are commonly generated by immunizing the host animal with pooled immunoglobulins from the target species and may be further purified or modified (e.g., immunoaffinity chromatography, antibody fragmentation, label conjugation) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Available Conjugate Formats
- Alexa Fluor 350
- Alexa Fluor 488
- Alexa Fluor 546
- Alexa Fluor 555
- Alexa Fluor 568
- Alexa Fluor 594
- Alexa Fluor 647
- Alexa Fluor 790
- Alexa Fluor Plus 405
- Alexa Fluor Plus 488
- Alexa Fluor Plus 555
- Alexa Fluor Plus 594
- Alexa Fluor Plus 647
- Alexa Fluor Plus 680
- Alexa Fluor Plus 800
- AP
- Biotin
- FITC
- HRP
- TRITC
- Custom conjugation available upon request
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