Thermo Fisher Scientific SF3A1 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

0.04-0.4 µg/mL

-

Immunohistochemistry (Paraffin) (IHC (P))

1:1,000-1:2,500

-

Immunocytochemistry (ICC/IF)

0.25-2 µg/mL

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein corresponding to Human SF3A1. Recombinant protein control fragment (Product #RP-91383). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:SF3A1,uniProtId:Q15459-1,ncbiNodeId:9606,antigenRange:44-218,antigenLength:793,antigenImageFileName:PA5-56334_SF3A1_Q15459-1_Rabbit.svg,antigenImageFileNamePDP:PA5-56334_SF3A1_Q15459-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2647174

Product Specific Information

Immunogen sequence: YPPPEVRNIV DKTASFVARN GPEFEARIRQ NEINNPKFNF LNPNDPYHAY YRHKVSEFKE GKAQEPSAAI PKVMQQQQQT TQQQLPQKVQ AQVIQETIVP KEPPPEFEFI ADPPSISAFD LDVVKLTAQF VARNGRQFLT QLMQKEQRNY QFDFLRPQHS LFNYFTKLVE QYTKI

Highest antigen sequence identity to the following orthologs: Mouse - 99%, Rat - 99%.

Target Information

This gene encodes subunit 1 of the splicing factor 3a protein complex. The splicing factor 3a heterotrimer includes subunits 1, 2 and 3 and is necessary for the in vitro conversion of 15S U2 snRNP into an active 17S particle that performs pre-mRNA splicing. Subunit 1 belongs to the SURP protein family; named for the SURP motifs that are thought to mediate RNA binding. Subunit 1 has tandemly repeated SURP motifs in its amino-terminal half while its carboxy-terminal half contains a proline-rich region and a ubiquitin-like domain. Binding studies with truncated subunit 1 derivatives demonstrated that the two SURP motifs are necessary for binding to subunit 3 while contacts with subunit 2 may occur through sequences carboxy-terminal to the SURP motifs. Alternative splicing results in multiple transcript variants encoding different isoforms.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.