Thermo Fisher Scientific RPS6KB1 Antibody Cocktail

Applications

Tested Dilution

Publications

Western Blot (WB)

1-5 µg/mL

-

Immunocytochemistry (ICC/IF)

60 µg/mL

-

ELISA (ELISA)

0.03 ng/mL

-

in situ PLA (PLA)

1:1200

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / Ig Mix

Class

Cocktail

Type

Antibody

Clone

1E10

Immunogen

RPS6KB1 (NP_003152, 416 a.a. approximately 525 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:p70 S6 Kinase,uniProtId:P23443-1,ncbiNodeId:9606,antigenRange:416-525,antigenLength:525,antigenImageFileName:H00006198-M02_p70_S6_Kinase_P23443-1_House_mouse.svg,antigenImageFileNamePDP:H00006198-M02_p70_S6_Kinase_P23443-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

See Label

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

no preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Sequence of this protein is as follows: PSVLESVKEK FSFEPKIRSP RRFIGSPRTP VSPVKFSPGD FWGRGASAST ANPQTPVEYP METSGIEQMD VTMSGEASAP LPIRQPNSGP YKKQAFPMIS KRPEHLRMNL

Target Information

RPS6KB1 (p70S6K) is a serine/threonine kinase with 2 non-identical kinase catalytic domains which phosphorylates several residues of the S6 ribosomal protein. p70 S6 kinase is predominantly localized in the cytoplasm, and is essential in growth factors regulated cell proliferation, pathways involving cell motility, such as metastases, the immune response, and tissue repair. p70 S6 kinase acts downstream of phosphoinositide (PI) 3-kinase, and its main physiological target is the S6 ribosomal protein, which is involved in upregulation of protein synthesis. The kinase activity of p70 S6 kinase leads to an increase in protein synthesis and cell proliferation. Amplification of the region of DNA encoding p70 S6 kinase and overexpression are seen in some breast cancer cell lines.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.