Thermo Fisher Scientific CDKN3 Monoclonal Antibody (OTI2E11), TrueMAB
CDKN3 단백질을 표적으로 하는 Mouse IgG1 단일클론 항체로, Western blot, IHC, ICC/IF, Flow Cytometry에 사용 가능. 동결건조 형태로 제공되며, 재구성 후 1 mg/mL 농도로 사용. 인산화 관련 연구 및 암 발현 분석에 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI2E11 |
| Immunogen | Full length human recombinant protein of human CDKN3 produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step (recommended: Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
The CDKN3 gene encodes a dual specificity protein phosphatase that acts as a cyclin-dependent kinase inhibitor. It interacts with and dephosphorylates CDK2 kinase, preventing its activation. This gene has been associated with various cancers due to deletion, mutation, or overexpression. Multiple alternatively spliced transcript variants encoding different isoforms have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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