Thermo Fisher Scientific Goat anti-Human IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 594
Alexa Fluor 594 형광으로 표지된 염소 유래 항-인간 IgG(H+L) 2차 항체로, WB, ICC/IF, Flow Cytometry에 적합. 인간 IgG에 특이적이며 마우스·토끼·소 혈청에 교차흡착 처리되어 높은 특이성과 낮은 비특이 결합을 제공. 연구용으로 사용.
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Thermo Fisher Scientific Goat anti-Human IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 594
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunocytochemistry (ICC/IF) | 1–10 µg/mL |
| Flow Cytometry (Flow) | 1–10 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Gamma Immunoglobins Heavy and Light chains |
| Conjugate | Alexa Fluor™ 594 |
| Excitation / Emission Max | 590 / 618 nm |
| Form | Liquid |
| Concentration | 2 mg/mL |
| Purification | Purified |
| Storage Buffer | PBS, pH 7.5 |
| Contains | 5 mM Sodium Azide |
| Storage Conditions | 4°C, store in dark |
| Shipping Conditions | Ambient |
| RRID | AB_2534081 |
| Target | IgG |
| Cross Adsorption | Against mouse, rabbit and bovine serum prior to conjugation |
| Antibody Form | Whole Antibody |
Additional Formats Available
- Alexa Fluor 488
- Alexa Fluor 546
- Alexa Fluor 555
- Alexa Fluor 568
- Alexa Fluor 633
- Alexa Fluor 647
- Alexa Fluor Plus 405
- Alexa Fluor Plus 488
- Alexa Fluor Plus 555
- Alexa Fluor Plus 594
- Alexa Fluor Plus 647
- AP
- Biotin
- FITC
- HRP
- Rhodamine
- TRITC
- Request custom conjugation
Product Specific Information
- Product will be shipped at room temperature.
Target Information
Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins. They are useful for detection, sorting, or purification of the specified target.
Secondary antibodies provide versatility for various detection systems (HRP, AP, fluorescence) and enhance sensitivity through signal amplification, as multiple secondary antibodies can bind to a single primary antibody.
Typically, secondary antibodies are generated by immunizing the host animal with pooled immunoglobulins from the target species and further purified or modified (e.g., immunoaffinity chromatography, antibody fragmentation, label conjugation) to yield highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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