Thermo Fisher Scientific ICSA Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

-

ELISA (ELISA)

Assay-dependent

-

Product Specifications

Species Reactivity

Bacteria

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant Shigella flexneri ICSA protein (311-547aa)

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.745 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.03% Proclin 300

Storage conditions

-20°C or -80°C if preferred

Shipping conditions

Wet ice

RRID

AB_3091654

Target Information

An immunogenic protein essential for bacterial spreading, it elicits polar deposition of filamentous actin (actin-based motility) (PubMed:2542950, PubMed:8702989). Inside the host (human) cell mediates nucleation and polymerization of actin molecules on the bacterial surface, which provides the propulsive force for intracellular movement and intercellular dissemination of the bacterium (PubMed:2644195, PubMed:8468279, PubMed:2542950). During invasion of mammalian cells, triggers autophagy by binding to host ATG5. Interaction with IcsB leads to escape from the host autophagy defense system (PubMed:15576571). Binds ATP and displays weak ATPase activity (PubMed:8468279). Mediates polar adhesion to host cells and their invasion. The bile salt deoxycholate activates adhesion and invasion of host cells, possibly by altering IcsA`s conformation. The adhesion and intercellular dissemination phenotypes can be separated (PubMed:24721572).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.