Thermo Fisher Scientific TMT Monoclonal Antibody (25D5)
TMT 단백질 표지용 항체로 Western blot 및 Immunoprecipitation에 최적화. Mouse IgG2b 단클론 항체(25D5)이며 Protein G로 정제됨. PBS + 4% BSA 보존액에 담긴 액상 형태로 -20°C 보관. 연구용으로만 사용 가능.
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000 | View 7 publications |
| Immunoprecipitation (IP) | Assay-dependent | - |
| Immunoelectrophoresis (IE) | - | View 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Chemical |
| Published Species | Chemical, Tag |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 25D5 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS with 4% BSA |
| Contains | Kathon |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10854708 |
Product Specific Information
- Product #90075 binds to immobilized TMT surface.
- Product #90075 has been successfully used in immunoprecipitation and Western blot applications.
Target Information
Tandem Mass Tag (TMT) reagents enable concurrent identification and multiplexed quantitation of proteins in different samples using tandem mass spectrometry (MS).
Isobaric TMT reagents share identical structures, including a peptide-reactive labeling group, a spacer arm, and an MS/MS reporter.
During MS/MS analysis, each isobaric tag produces a unique reporter ion that enables quantitation.
Amine-reactive NHS-ester isotopic duplex or six-plex isobaric TMT tags covalently react with amino termini and lysine residues of peptides and proteins.
Cysteine-reactive Tandem Mass Tag (cysTMT) reagents react with reduced thiols of cysteine-containing peptides.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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