Thermo Fisher Scientific TMT Monoclonal Antibody (25D5)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 7 publications 7 publications

Immunoprecipitation (IP)

Assay-dependent

-

Immunoelectrophoresis (IE)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Chemical

Published species

Chemical, Tag

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

25D5

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.1 mg/mL

Purification

Protein G

Storage buffer

PBS with 4% BSA

Contains

Kathon

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10854708

Product Specific Information

Product # 90075 binds to immobilized TMT surface.

Product # 90075 has been succesfully used in immunoprecipitation and Western blot applications.

Target Information

Tandem Mass Tag (TMT) reagents enable concurrent identification and multiplexed quantitation of proteins in different samples using tandem mass spectrometry (MS). Isobaric TMT reagents share identical strucures, including a peptide-reactive labeling group, a spacer arm and a MS/MS reporter. During the MS/MS analysis, each isobaric tag produces a unique reporter ion that makes quantitation possible. Amine-reactive NHS-ester isotopic duplex or six-plex isobaric TMT tags covalently react with amino termini and lysine residues of peptides and proteins. Cysteine-reactive Tandem Mass Tag (cysTMT) reagents react with reduced thiols of cysteine-containing peptides.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.